APOB Polyclonal Antibody

Catalogue Numbers: AP6031-50, AP6031-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04114

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB-100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: APOB polyclonal antibody detects endogenous levels of APOB protein.

Extra Notes: Western blot (WB) analysis of APOB polyclonal antibody at 1:1000 dilution
Lane1:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB(10ng)
Lane2:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB.(20ng)
Lane3:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB.(50ng)

Bioworld Molecular Weight: ~ 516 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Apolipoprotein B-100; Apo B-100

Immunogen: Recombinant APOB protein, corresponding to amino acids 97-526 of Human APOB.

Conjugate: Unconjugated

Modification: Unmodified