HSD3B2 Polyclonal Antibody

Catalogue Numbers: BS60084-50, BS60084-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P26439

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: 3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD is the type I protein that catalyzes the oxidative conversion of ∂5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3β-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3β-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSD3B2 polyclonal antibody detects endogenous levels of HSD3B2 protein.

Extra Notes: Western blot (WB) analysis of HSD3B2 pAb at 1:500 dilution
Lane1:A2780 whole cell lysate(30ug)
Lane2:SK-OVCAR3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 3β-HSD; HSD-3β; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3-beta-HSD II; 3-beta-HSD adrenal and gonadal type; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase; HSD3B2; HSDB3B

Immunogen: A synthetic peptide corresponding to residues in Human HSD3B2.

Conjugate: Unconjugated

Modification: Unmodified