PLSCR1 Polyclonal Antibody

Catalogue Numbers: BS60203-50, BS60203-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15162

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The calcium-dependent plasma membrane protein phospholipid scramblase 1 (PLSCR1) contributes to the transbilayer movement of phosphatidylserine and other membrane phospholipids upon influx of calcium into the cytosol. This movement results in plasma membrane phospholipid remodelling and surface exposure of phosphatidylserine in injured or apoptotic cells, which leads to cell death. Interferons and other cytokines induce expression of PLSCR1, implying that PLSCR1 also functions in cytokine signaling pathways. EGF stilmulation results in tyrosine phosphorylation of PLSCR1 on tyrosines 69 and 74, which allows it to interact with Shc, and thereby connecting Src kinase activation to stimulation of the EGF receptor.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PLSCR1 polyclonal antibody detects endogenous levels of PLSCR1 protein.

Extra Notes: Western blot (WB) analysis of PLSCR1 pAb at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:CT26 whole cell lysate(40ug)
Lane3:HepG2 whole cell lysate(40ug)
Lane4:HCT116 whole cell lysate(40ug)
Lane5:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Phospholipid scramblase 1; PL scramblase 1; Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b

Immunogen: A synthetic peptide corresponding to residues in Human PLSCR1.

Conjugate: Unconjugated

Modification: Unmodified