Recombinant Human HMGB3 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032546-10, PKSH032546-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: HMGB3

Target Synonym: High Mobility Group Protein B3; High Mobility Group Protein 2a; HMG-2a; High Mobility Group Protein 4; HMG-4; HMGB3; HMG2A; HMG4

UNIProt ID: O15347

Research Areas: epigenetics and nuclear signal

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: O15347

Background: High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important role in DNA replication, nucleosome assembly and transcription. HMGB3 binds preferentiallly single-stranded DNA and unwinds double stranded DNA. HMGB3 consists of 200 amino acids and is localized to the cell nucleus. It contains two HMG box DNA-binding domain. HMGB3 binds preferentially single-stranded DNA and unwinds double stranded DNA.

Sequence: Met 1-Glu200

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 24.0 kDa

Observed MW: 26 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only