Raf1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16471-50, BZ16471-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P04049

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody., Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The Lane on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody, and DAPI.

Alternative Name: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300

Conjugate: Unconjugated

Modification: Unmodified