Malic Acid Colorimetric Assay Kit

Size: 96T

Catalogue Number: E-BC-K905-M-96

Citations, Manuals and MSDS Available upon request.

Research Areas: Tricarboxylic Acid（TCA）Cycle

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum (plasma) , tissue and cell

Assay Time: 50 min

Detection Type: Colorimetric method

Sensitivity: 0.003 mmol/L

Detection Range: 0.003-0.5 mmol/L

Test Principle: L-malic acid, also known as 2-hydroxysuccinic acid, is widely present in living organisms. It has two stereoisomers and is one of the members of the tricarboxylic acid cycle, an important metabolic cycle in living organisms. The detection principle of this kit is that the enzyme catalyzes malic acid to produce oxaloacetic acid. At the same time, under the action of electronic coupler, the chromogenic agent is reduced to produce orange yellow product, which has the maximum absorption peak at about 450 nm.

Inter CV: 4.6-5.9

Intra CV: 2.6-3.6

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag