PGRMC2 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS76687-50, BS76687-100

Swiss-Prot: O15173

Host: Rabbit

PGRMC2 Polyclonal Antibody-BS76687

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity. May serve as a universal non-classical progesterone receptor in the uterus (Probable. Intracellular heme chaperone required for delivery of labile, or signaling heme, to the nucleus (By similarity. Plays a role in adipocyte function and systemic glucose homeostasis. In brown fat, which has a high demand for heme, delivery of labile heme in the nucleus regulates the activity of heme-responsive transcriptional repressors such as NR1D1 and BACH1 (By similarity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 23kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of MCF-7 cells, using PGRMC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min., Immunofluorescence analysis of NIH/3T3 cells using PGRMC2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PGRMC2; DG6; PMBP

Immunogen: A synthetic peptide of human PGRMC2(NP_006311.3).

Modification: Unmodified

Conjugate: Unconjugated