IKKα/β (phospho-S176/177) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4236-50, BS4236-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15111/O14920

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-IKKα/β (S176/177) polyclonal antibody detects endogenous levels of IKKα/β protein only when phosphorylated at Ser176/177.

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IKKα/β (phospho-S176/177) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: HEK293T treated with TNFα(20ng/ml)for 10 minutes whole cell lysate
Lane 3: HEK293T treated with TNFα(20ng/ml)for 40 minutes whole cell lysate
Lane 4: HEK293T treated with TNFα(20ng/ml)for 1 hour whole cell lysate, Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of p-IKKα/β (S176/177) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; EC 2.7.11.10; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK; IKKA; TCF16

Immunogen: Synthetic phosphopeptide derived from human IKKα/β around the phosphorylation site of Serine 176/177.

Conjugate: Unconjugated

Modification: Phosphorylation