Very fast purification and/or concentration of PCR products, between 60 bp and 30 kb length, through simple but effective spin column technique.

The high performance membrane used for the Roti^®Prep PCR Purification columns, allows purification or concentration of PCR products within approx. 3 minutes. The PCR product to be extracted (50 µl in maximum) is first mixed with binding buffer, which can be done separately or directly in the spin filter column. The nucleic acid is then eluted by centrifugation, without any additional washing step, in a minimum of 10 µl of water or buffer.Isolated DNA is free of residual nucleotides or PCR additives, and may directly be applied to all standard downstream applications like cloning or sequencing.

Binding Buffer, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.Contents of this Kit may not be bought separately.